Benzalkonium Chloride Suppresses Rabbit Corneal Endothelium Intercellular Gap Junction Communication
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چکیده
منابع مشابه
Benzalkonium Chloride Suppresses Rabbit Corneal Endothelium Intercellular Gap Junction Communication
PURPOSE Gap junction intercellular communication (GJIC) plays a critical role in the maintenance of corneal endothelium homeostasis. We determined if benzalkonium chloride (BAK) alters GJIC activity in the rabbit corneal endothelium since it is commonly used as a drug preservative in ocular eyedrop preparations even though it can have cytotoxic effects. METHODS Thirty-six adult New Zealand al...
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788 with a lipidlprotein molar ratio of 100:1, a value close to that found in most biological membranes [21]. The data show that the calculation of distances between sites within the monomer of a membrane protein of a cross-sectional diameter of 5 nm based only from FET efficiency measurements is largely speculative in most cases in native biological membranes. Because the contribution of FET t...
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PURPOSE Keratocytes are connected to each other by gap junctions, which mediate intercellular communication and contribute to maintenance of corneal homeostasis. The possible effect of tumor necrosis factor (TNF)-alpha, a proinflammatory cytokine, on gap junctional intercellular communication (GJIC) in cultured human corneal fibroblasts was examined. METHODS GJIC activity was measured by obse...
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RESULTS. TNFinduced a timeand concentration-dependent decrease in GJIC activity in human corneal fibroblasts. Immunofluorescence analysis revealed that TNFreduced the level of specific staining for Cx43 at sites of contact between adjacent cells. Immunoblot analysis detected four specific Cx43 bands, one corresponding to the nonphosphorylated form of the protein and three corresponding to phosp...
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PURPOSE The aim of this study was to determine and characterize the effect of topical application of benzalkonium chloride (BAK) on corneal nerves in vivo and in vitro. METHODS Thy1-YFP+ neurofluorescent mouse eyes were treated topically with vehicle or BAK (0.01% or 0.1%). Wide-field stereofluorescence microscopy was performed to sequentially image the treated corneas in vivo every week for ...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2014
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0109708